Prabin Dhangada Majhi, PhD

661 N Pleasant St. Amherst MA | 413-548-0077 | prabin.dm2@gmail.com linkedin.com/in/prabindm/ | Google Scholar: http://bit.ly/2Q6P8BI Work Authorization: U.S. Permanent Resident

Professional Summary

Molecular biologist and genomics scientist with 15+ years of post-PhD experience designing and executing multi-modal NGS workflows, variant analysis pipelines, and functional molecular assays. Expertise spans bulk RNA-Seq, scRNA-Seq, spatial transcriptomics, hybrid-capture target enrichment, INDUCE-Seq (double-strand break sequencing), and NGS variant analysis leveraging GTEx and 1000 Genomes reference datasets. Proven ability to lead multi-project assay development programs, author technical documentation, mentor scientists, and coordinate across bioinformatics, molecular biology, and translational teams. Authorized to work in the U.S. without sponsorship.

Relevant Skills

NGS Workflows & Assay Development: Bulk RNA-Seq library preparation | Single-cell RNA-Seq (library prep, cell isolation, sequencing) | Spatial Transcriptomics | Hybrid capture target enrichment | INDUCE-Seq (DSB sequencing) | DNA/RNA isolation from blood, tissue, primary cells | qRT-PCR | PCR primer design | Cloning

Variant Analysis & Bioinformatics: NGS variant analysis pipelines | GTEx expression quantitative trait locus (eQTL) analysis | 1000 Genomes population variant interpretation | RNA-Seq differential expression | scRNA-Seq (Seurat, cell type annotation) | R | Python | Shell | HPC and Google Cloud platforms | CBioPortal | DepMap | AllofUS research workbench

Molecular Biology & Functional Assays: CRISPR/Cas9 genome editing | Western blot | Co-IP | Immunoprecipitation | Immunofluorescence | Immunohistochemistry | Multiplex immunofluorescence | Luminex | Flow cytometry (10+ color, BD Fortessa, LSR, Imagestream) | FACS-based cell sorting

Research Leadership & Documentation: SOP and protocol authorship | Technical report and manuscript writing | Multi-project program leadership | Scientist mentorship and training | IRB/ethics protocol management | ELN and GLP documentation

Education

PhD, Bioscience and Bioengineering Indian Institute of Technology Bombay (IITB), Mumbai, India — 2010

MSc, Life Sciences Utkal University, Bhubaneswar, India — 2002

Professional Experience

Research Assistant Professor

University of Massachusetts, Amherst MA | 2020–Present

• Designed and executed multi-modal NGS workflows including bulk RNA-Seq, scRNA-Seq, spatial transcriptomics, and hybrid-capture target enrichment sequencing; performed NGS variant analysis leveraging GTEx eQTL datasets and 1000 Genomes population frequency data to interpret genomic variants in the context of hormone signaling-induced DNA damage.

• Developed and applied INDUCE-Seq (double-strand break sequencing) to map genome-wide DSB landscapes at nucleotide resolution, establishing library preparation protocols, target enrichment strategies, and bioinformatics pipelines from scratch — demonstrating end-to-end NGS assay development capability.

• Established a biobank of 16 patient-derived immortalized breast epithelial cell lines from tissue samples (reduction mammoplasty and mastectomy), encompassing DNA/RNA extraction, library preparation, and NGS profiling across a diverse sample repository — directly relevant to rare disease diagnostic sample processing workflows.

• Designed and implemented an automated high-throughput imaging and analysis platform integrating machine learning segmentation (Ilastik), CellProfiler, and NIKON AR Elements, establishing performance specifications, validation procedures, and QC metrics — reflecting the assay development lifecycle from design through V&V.

• Secured NCI R03 funding; coordinated multi-investigator programs across three independent research groups with full responsibility for study design, timelines, data deliverables, and technical reporting; supervised and mentored graduate students and postdoctoral researchers.

Research Scientist

University of Massachusetts, Amherst MA | 2016–2020

• Performed functional characterization of DNA damage response variants using qRT-PCR, Western blot, and immunofluorescence assays (γH2AX, 53BP1, R-loop) across primary breast epithelial cells and mouse models; applied GTEx and 1000 Genomes datasets to contextualize variant frequencies and expression patterns in normal tissue, published in Environ Health Persp (2020) and Oncogene (2021).

• Developed and validated DNA fiber assay and R-loop immunofluorescence protocols as novel research tools, authoring SOPs and training junior researchers — directly reflecting the assay development and documentation standards required in a diagnostics setting.

Post-doctoral Researcher

University of Nebraska Medical Center, Omaha, NE | 2010–2012

• Applied molecular cloning, site-directed mutagenesis, Co-IP, and cell fractionation to characterize MUC16 proteolytic processing and chromatin localization; generated stable overexpression and knockdown cell lines and performed comprehensive functional characterization across 5 publications (Clin Cancer Res, Sci. Reports, Oncotarget, J Thorac Oncol, 2013–2017).

Assistant Professor

Ravenshaw University & Delhi University, India | 2012–2022

Awards

• Raman Post-doctoral Fellowship — 2016
• Career-MODE Fellowship — 2022
• UMASS FRG/Healy Research Grant — 2023

Select Publications

17 peer-reviewed publications ( Co-first author, @ Co-lead author)*

• Majhi PD*@ et al. Inducible estrogen receptor alpha in normal breast epithelial cells demonstrate estrogen receptor-dependent DNA damage. bioRxiv (2025). 10.1101/2025.03.13.643100

• Majhi PD et al. Genetic modifiers regulating DNA replication and double-strand break repair in Li-Fraumeni mammary tumor models. Oncogene 40, 5026–5037 (2021). 10.1038/s41388-021-01892-5

• Majhi PD* et al. Effects of Benzophenone-3 and Propylparaben on Estrogen Receptor-Dependent R-Loops and DNA Damage. Environ Health Persp 128, 17002 (2020). 10.1289/ehp5221

• Majhi PD* et al. Pathobiological Implications of MUC4 in NSCLC. J Thorac Oncol 8, 398–407 (2013). 10.1097/jto.0b013e3182829e06

Patent (Pending — Notice of Allowance Received)

Majhi PDK, Sharma A, Jerry DJ. “Methods for Predicting Estrogen Receptor-Mediated DNA Damage.” U.S. Serial No. 62/914,110. Notice of Allowance received February 2026. (Patent number pending issuance.)